Historique
Article de Périodique
Survival of hepatitis C virus in syringes: implication for transmission among injection drug users (2010)
Auteur(s) :
PAINTSIL, E ;
HE, H. ;
PETERS, C ;
LINDENBACH, B. D. ;
HEIMER, R.
Année :
2010
Page(s) :
984-990
Langue(s) :
Anglais
Refs biblio. :
48
Domaine :
Drogues illicites / Illicit drugs
Discipline :
MAL (Maladies infectieuses / Infectious diseases)
Note générale :
Editorial commentary: "The beginning of a new era in understanding hepatitis C virus prevention", J.D. Rich, L.E. Taylor, p.981-983.
Résumé :
Background. We hypothesized that the high prevalence of hepatitis C virus (HCV) among injection drug users might be due to prolonged virus survival in contaminated syringes.
Methods. We developed a microculture assay to examine the viability of HCV. Syringes were loaded with blood spiked with HCV reporter virus (Jc1/GLuc2A) to simulate 2 scenarios of residual volumes: low void volume (2 µL) for 1‐mL insulin syringes and high void volume (32 μL) for 1‐mL tuberculin syringes. Syringes were stored at 4°C, 22°C, and 37°C for up to 63 days before testing for HCV infectivity by using luciferase activity.
Results.The virus decay rate was biphasic (t(1/2)alpha = 0.4 h and t(1/2)beta = 28 h). Insulin syringes failed to yield viable HCV beyond day 1 at all storage temperatures except 4°, in which 5% of syringes yielded viable virus on day 7. Tuberculin syringes yielded viable virus from 96%, 71%, and 52% of syringes after storage at 4°, 22°, and 37° for 7 days, respectively, and yielded viable virus up to day 63.
Conclusions. The high prevalence of HCV among injection drug users may be partly due to the resilience of the virus and the syringe type. Our findings may be used to guide prevention strategies. [Author's abstract]
Methods. We developed a microculture assay to examine the viability of HCV. Syringes were loaded with blood spiked with HCV reporter virus (Jc1/GLuc2A) to simulate 2 scenarios of residual volumes: low void volume (2 µL) for 1‐mL insulin syringes and high void volume (32 μL) for 1‐mL tuberculin syringes. Syringes were stored at 4°C, 22°C, and 37°C for up to 63 days before testing for HCV infectivity by using luciferase activity.
Results.The virus decay rate was biphasic (t(1/2)alpha = 0.4 h and t(1/2)beta = 28 h). Insulin syringes failed to yield viable HCV beyond day 1 at all storage temperatures except 4°, in which 5% of syringes yielded viable virus on day 7. Tuberculin syringes yielded viable virus from 96%, 71%, and 52% of syringes after storage at 4°, 22°, and 37° for 7 days, respectively, and yielded viable virus up to day 63.
Conclusions. The high prevalence of HCV among injection drug users may be partly due to the resilience of the virus and the syringe type. Our findings may be used to guide prevention strategies. [Author's abstract]
Affiliation :
Departments of Pediatrics and Pharmacology, Yale University School of Medicine, 333 Cedar St, New Haven, CT 06520, United States / Etats-Unis
Cote :
A02248