Titre : | HIV-1 genetic diversity in Galicia spain: BG intersubtype recombinant viruses circulating among injecting drug users |
Titre traduit : | (Diversité génétique du VIH-1 en Galicie espagnole : circulation de virus recombinants d'inter-sous type BG parmi les usagers de drogue injectable.) |
Auteurs : | M. M. THOMSON ; E. DELGADO ; MANJON N. ; OCAMPO A. ; VILLAHERMOSA M. L. ; MARINO A. ; HERRERO I. ; CUEVAS M. T. ; VASQUEZ-DE PARGA E. ; L. PEREZ-ALVAREZ ; MEDRANO L. ; J. A. TABOADA ; NAJERA R. ; THE SPANISH GROUP FOR ANTIRETROVIRAL STUDIES IN GALICIA |
Type de document : | Périodique |
Année de publication : | 2001 |
Format : | 509-516 |
Note générale : |
AIDS, 2001, 15, (4), 509-516
|
Langues: | Anglais |
Discipline : | MAL (Maladies infectieuses / Infectious diseases) |
Mots-clés : |
Thésaurus mots-clés VIH ; INJECTION ; CONTAMINATION ; GENETIQUE ; EPIDEMIOLOGIE DESCRIPTIVEThésaurus géographique ESPAGNE |
Résumé : |
FRANÇAIS :
En Europe de l'Ouest, les virus de sous type B sont principalement responsables de l'épidémie à VIH-1, les autres sous types étant rares. Chez 451 personnes (de 7 villes de Galicie), les séquences ARN des sous types ont été déterminées selon l'algorithme de Blast. 4,4% de virus non de sous type B, avec une grande diversité de formes génétiques, ont été identifiés. 12 des infections, non dues à des virus de sous type B (soit 60%) concernaient les usagers de drogues injectables, qui pour la plupart n'avaient connaissance d'aucune source de contamination hors de l'Europe. ENGLISH: Background: The HIV-1 epidemics in Western Europe are dominated by B subtype viruses. Non-B subtype is largely restricted to individuals infected outside of Europe and to their direct contacts and is generally acquired by the heterosexual route. Methods: Protease and a segment of reverse transcriptase were amplified and sequenced from plasma RNA in 451 individuals from seven cities of Galicia, north- western Spain. Subtype sequence homologies were determined using the BLAST algorithm. Non-B sequences were examined by phylogenetic analysis and intersubtype recombination by bootscanning. The env V3 region was analysed in all non-B and in 38 B subtype viruses. Results: Ten different non-B genetic forms were identified in 20 (4.4%) individuals. Subtypes were concordant between pol and V3 in five viruses; 14 (70%) infections were with intersubtype recombinant viruses, and one individual had a dual B+G infection. Seven recombinant viruses were phylogeneticaliy related to five reported recombinant forms. Three non-recombinant G and six recombinant BG viruses formed a monophyletic cluster for pol. All but three individuals with non-B infections were native Spanish. Only 6 of 1 6 individuals referred to sexual contacts with sub-Saharan Africans. Twelve (60%) non-B subtype infections, including all with G and BG viruses, were in injecting drug users (IDU). Conclusions: Non-B subtype viruses were identified in 4.4%, with a high diversity of genetic forms, including 70% infections with intersubtype recombinant viruses. The majority of individuals with non-B infections were IDU, most of them without known contacts with non-European sources, and among whom BG recombinant viruses are circulating. (Author' s abstract) |
Domaine : | Drogues illicites / Illicit drugs |
Affiliation : |
Ctro Nacional Biologia Fundamental, Insto Salud Carlos III, Ctra Majadahonda-Pozuelo, 28220 Majadahonda (Madrid) Espagne. Spain. |
Numéro Toxibase : | 205657 |
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