Périodique
HIV-specific CD8 T-cell activity in uninfected injection drug users is associated with maintenance of seronegativity
(L'activité spécifique vis-à-vis du VIH des cellules CD8 T chez les usagers de drogues non infectés est associée au maintien de la séronégativité.)
Auteur(s) :
MAKEDONAS G. ;
J. BRUNEAU ;
H. LIN ;
SEKALY R. P. ;
F. LAMOTHE ;
N. F. BERNARD
Article en page(s) :
15-5-1602
Refs biblio. :
31
Domaine :
Drogues illicites / Illicit drugs
Langue(s) :
Anglais
Discipline :
MAL (Maladies infectieuses / Infectious diseases)
Note générale :
AIDS, 2002, 16, (12), 15-5-1602
Note de contenu :
fig. ; tabl.
Résumé :
ENGLISH :
Objectives: To determine whether HIV-exposed, uninfected subjects (EUs) having HIV-specific effector activity are at a reduced risk for seroconverting compared with EUs with no HIV-specific effector responses. Design: Twenty-eight intravenous drug users (IVDU) with documented risk for HIV infection over a 1-year period were screened for the presence of HIV-specific CD8+ effector cell activity. Group I included 18 IVDUs who remained seronegative despite exposure to HIV through needle sharing with partner(s) known to be HIV infected. Group II included 10 IVDUs who seroconverted after similar HIV exposure. Methods: The enzyme-linked immunospot (ELlspot; Mabtech AB, Nacka, Sweden) assay was used to measure the frequency of HIV-specific interferon-y secreting cells. Peripheral blood mononuclear cells (PBMC) were stimulated with a panel of synthetic HIV peptides in a major histocompatibility complex class I antigen restricted fashion. PBMC from group II were obtained from timepoints 7 months or less before seroconversion. Results: Twelve of 18 (66.7%) persistently seronegative subjects versus none of 10 seroconverters exhibited detectable HIV-specific effector responses at the sampling date (P 3000). Conclusion: EUs who have developed H1V-specific effector responses are at a reduced risk for seroconversion compared with EUs who do not develop this type of immunity. This observation supports the hypothesis that HIV-specific effector responses are a correlate of immune protection from HIV infection. (Editor' s abstract)
ENGLISH :
Objectives: To determine whether HIV-exposed, uninfected subjects (EUs) having HIV-specific effector activity are at a reduced risk for seroconverting compared with EUs with no HIV-specific effector responses. Design: Twenty-eight intravenous drug users (IVDU) with documented risk for HIV infection over a 1-year period were screened for the presence of HIV-specific CD8+ effector cell activity. Group I included 18 IVDUs who remained seronegative despite exposure to HIV through needle sharing with partner(s) known to be HIV infected. Group II included 10 IVDUs who seroconverted after similar HIV exposure. Methods: The enzyme-linked immunospot (ELlspot; Mabtech AB, Nacka, Sweden) assay was used to measure the frequency of HIV-specific interferon-y secreting cells. Peripheral blood mononuclear cells (PBMC) were stimulated with a panel of synthetic HIV peptides in a major histocompatibility complex class I antigen restricted fashion. PBMC from group II were obtained from timepoints 7 months or less before seroconversion. Results: Twelve of 18 (66.7%) persistently seronegative subjects versus none of 10 seroconverters exhibited detectable HIV-specific effector responses at the sampling date (P 3000). Conclusion: EUs who have developed H1V-specific effector responses are at a reduced risk for seroconversion compared with EUs who do not develop this type of immunity. This observation supports the hypothesis that HIV-specific effector responses are a correlate of immune protection from HIV infection. (Editor' s abstract)
Affiliation :
1650 Cedar Ave., Rm C10-160, Montréal, Quebec
Canada. Canada.
Canada. Canada.
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