Historique
Périodique
Rapid screening method for determination of ecstasy and amphetamines in urine samples using gas chromatography-chemical ionisation mass spectrometry
(Méthode rapide de criblage pour la détection d'ecstasy et d'amphétamines dans des échantillons d'urine par chromatographie gazeuse et spectrométrie de masse à ionisation chimique)
Auteur(s) :
PELLEGRINI, M. ;
ROSATI F. ;
PACIFICI, R. ;
ZUCCARO, P. ;
ROMOLO F. S. ;
LOPEZ, A.
Année
2002
Page(s) :
243-251
Langue(s) :
Français
Refs biblio. :
24
Domaine :
Drogues illicites / Illicit drugs
Thésaurus mots-clés
MDMA-ECSTASY
;
AMPHETAMINE
;
DEPISTAGE
;
URINE
;
BIOCHIMIE
;
ANALYSE CHIMIQUE
;
MDA
;
MDEA
Thésaurus géographique
EUROPE
Note générale :
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2002, 769, (2), 243-251
Note de contenu :
graph. ; tabl.
Résumé :
ENGLISH :
The need for analytical screening tests more reliable and valid to detect amphetamine and related "designer drugs" in biological samples is becoming critical, due to the increasing diffusion of these drugs on the European illegal market. The most common screening procedures based on immunoassays suffer a number of limitations, including low sensitivity, lack of specificity and limited number of detectable substances. This paper describes a screening method based on gas-chromatography-mass-spectrometry (GC/MS) using positive chemical ionisation (PCI) detection. Methanol was used as reactant gas in the ionisation chamber. Molecular ions of different compounds were monitored, allowing a sensitivity of 5-10 ng/ml with high selectivity. The sensitivity of the method gives positive results in samples taken 48-72 h after intake of one dose of 50-100 mg. The method is simple and rapid. Sample preparation was limited to one liquid-liquid extraction, without any hydrolysis and derivatisation. Hydrolysis is critical to identify metabolites excreted as conjugates. Blank urine samples spiked with known amounts of amphetamine (AM), methylamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethylamphetamine (MDMA), methylenedioxyethylamphetamine (MDEA) and methylenedioxyphenyl-N-methyl-2-butanamine (MBDB) were analysed. The method was successfully tested on real samples of urine from people, whose use of amphetamine was suspected, and results were compared with results obtained with immunoassays. (Review' s abstract)
Affiliation :
Clinical Biochemistry Dpt, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome
Italie. Italy.
Italie. Italy.
Cote :
A01135