Historique
Périodique
Antiestrogenic effects of marijuana smoke condensate and cannabinoid compounds
(Effets antioestrogènes du condensé de fumée de marijuana et des composés cannabinoïdes.)
Auteur(s) :
LEE, S. Y. ;
OH S. M. ;
LEE, S. K. ;
CHUNG, K. H.
Année
2005
Page(s) :
1365-1375
Langue(s) :
Français
Refs biblio. :
46
Domaine :
Drogues illicites / Illicit drugs
Discipline :
PRO (Produits, mode d'action, méthode de dépistage / Substances, action mode, screening methods)
Thésaurus mots-clés
CANNABIS
;
REPRODUCTION
;
HORMONES
;
CANNABINOIDES
;
BIOLOGIE
;
USAGE RECREATIF
;
ENDOCRINOLOGIE
Note générale :
Archives of Pharmacal Research, 2005, 28, (12), 1365-1375
Résumé :
ENGLISH :
The antiestrogenic effects of marijuana smoke condensate (MSC) and three major cannabinoids, ie., Delta9-tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN), were evaluated using in vitro bioassays, viz., the human breast cancer cell proliferation assay, the recombinant human estrogen receptor (ER) competitive binding assay, and the reporter gene assay. The inhibitory effects on estrogen were also examined using the ethoxyresorufin-O-deethylase (EROD) assay, the aromatase assay, and the 17ß-estradiol (E2) metabolism assay. The results showed that MSC induced the antiestrogenic effect via the ER-mediated pathway, while THC, CBD, and CBN did not have any antiestrogenic activity. This suggests that the combined effects of the marijuana smoke components are responsible for the antiestrogenicity of marijuana use. In addition, MSC induced the CYP1A activity and the E2 metabolism, but inhibited the aromatase activity, suggesting that the antiestrogenic activity of MSC is also related to the indirect ER-dependent pathway, as a result of the depletion of the in situ E2 level available to bind to the ER. In conclusion, pyrogenic products including polycyclic aromatic hydrocarbons (PAHs) in the non-polar fraction, which is the most biologically active fraction among the seven fractions of MSC, might be responsible for the antiestrogenic effect. (Author' s abstract)
Affiliation :
National Institute of Scientific Investigation, Seoul
Corée du Sud. South Korea.
Corée du Sud. South Korea.
Cote :
A03178