Historique
Article de Périodique
Evaluation of immunoassay methods for the screening of cocaine metabolites in urine (1991)
(Evaluation des techniques immunologiques de recherche des métabolites de la cocaïne dans l'urine)
Auteur(s) :
BAUGH, L. D. ;
ALLEN, E. E. ;
LIU, R. H. ;
LANGNER, J. G. ;
FENTRESS J.C. ;
CHADHA S.C. ;
COOK, L. F. ;
WALIA A.S.
Année
1991
Page(s) :
79-85
Langue(s) :
Anglais
Refs biblio. :
8
Domaine :
Drogues illicites / Illicit drugs
Discipline :
PRO (Produits, mode d'action, méthode de dépistage / Substances, action mode, screening methods)
Résumé :
FRANÇAIS :
La comparaison sur 62 échantillons de deux kits de détermination urinaire de la cocaïne et de son métabolite principal, la benzoylecgonine, utilisant la technique EMIT à une technique couplant CPG et MS, montre que chaque produit a ses propres avantages et inconvénients, mais que le test TDx* est plus approprié que le test Abuscreen* quant à la détermination de la benzoylecgonine.
ENGLISH :
Immunoassay kits for urine cocaine (and metabolite) screening, obtained from two commercial sources, were examined for correlation of their results, expressed in terms of equivalent benzoylecgonine concentration, with the gas chromatography/mass spectrometry (GC/MS) concentration of benzoylecgonine. The correlation coefficients obtained, based on 62 (out of a total sample population of 3295) highly relevant samples, were 0.467 and 0.766 for Abuscreen (ARIA) and TDx (TDX), respectively. The preliminary screen cutoff values, which correspond to 150 ng/mL benzoylecgonine (as determined by GC/MS), were calculated based on the resulting regression equations and found to be 380 and 190 ng/mL for ARIA and TDX, respectively. With these cutoff values, ARIA generates 5 false negatives and 16 unconfirmed presumptive positives, while TDX results in 3 false negatives and 6 unconfirmed presumptive positives.
La comparaison sur 62 échantillons de deux kits de détermination urinaire de la cocaïne et de son métabolite principal, la benzoylecgonine, utilisant la technique EMIT à une technique couplant CPG et MS, montre que chaque produit a ses propres avantages et inconvénients, mais que le test TDx* est plus approprié que le test Abuscreen* quant à la détermination de la benzoylecgonine.
ENGLISH :
Immunoassay kits for urine cocaine (and metabolite) screening, obtained from two commercial sources, were examined for correlation of their results, expressed in terms of equivalent benzoylecgonine concentration, with the gas chromatography/mass spectrometry (GC/MS) concentration of benzoylecgonine. The correlation coefficients obtained, based on 62 (out of a total sample population of 3295) highly relevant samples, were 0.467 and 0.766 for Abuscreen (ARIA) and TDx (TDX), respectively. The preliminary screen cutoff values, which correspond to 150 ng/mL benzoylecgonine (as determined by GC/MS), were calculated based on the resulting regression equations and found to be 380 and 190 ng/mL for ARIA and TDX, respectively. With these cutoff values, ARIA generates 5 false negatives and 16 unconfirmed presumptive positives, while TDX results in 3 false negatives and 6 unconfirmed presumptive positives.
Affiliation :
Department of Criminal Justice, University of Alabama, Birmingham, USA