Historique
Périodique
Prevalencia de anticuerpos anti-Leishmania en individuos adictos a drogas por via parenteral. Rentabilidad de dos técnicas de estudio
(Prévalence d'anticorps anti-Leishmaniose chez des toxicomanes par voie intraveineuse. Efficacité de deux techniques de dépistage) ; (Prevalence of antibodies anti-Leishmania in travenous drug addicts. Profitability of two study techniques)
Auteur(s) :
GUTIERREZ, J. ;
MAROTO M. C. ;
PIEDROLA G. ;
HIGUERA A.
Année
1993
Page(s) :
168-170
Langue(s) :
Espagnol
ISBN :
0025-7753
Refs biblio. :
21
Domaine :
Drogues illicites / Illicit drugs
Discipline :
PAT (Pathologie organique / Organic pathology)
Note générale :
Medicina Clinica , 1993, 100, (5), 168-170
Résumé :
FRANÇAIS :
Etude du dépistage des anticorps anti-Leishmaniose chez des patients UDVI suspectés de Leishmaniose viscerale, présentant des troubles fébriles et des adénopathies, comparativement à un groupe de sujets sains non UDVI. Les deux techniques utilisées sont d'une part, l'immunofluorscence indirecte pour détecter les anticorps IgG et IgM et d'autre part, le test Elisa pour l'analyse des anticorps IgG. Dans ce diagnostic indirecte de la Leishmaniose chez les UDVI les deux méthodes sont complémentaires, la séroconversion IgG peut se manifester lors d'affection aiguë sans s'accompagner d'une positivité des IgM.
ENGLISH :
The prevalence of antibodies anti-Leishmania in intravenous drug addict (IVDA) patients suspect of visceral leishmaniasis (VL) and the diagnostic profitability of two tests for detecting anti-Leishmania antibodies were studied. METHODS : Serum samples corresponding to 91 healthy non IVDA individuals (group 1), 143 IVDA individuals attended in consultation for febrile illness and lymph node enlargement (group 2) with only one serum determination, and 42 IVDA individuals in whom sequential serologic study (group 3) was performed were analyzed. IgG and IgM antibodies were studied by indirect immunofluorescence (IIF). The individuals of group 1 and 90 of those of group 2 (45 with anti-Leishmania IgG antibodies and 45 without by IIF) were simultaneously studied by ELISA to analyze IgG antibodies. It was found that indirect immunofluorescence and ELISA techniques are complementary methods in the indirect diagnosis of visceral leishmaniasis overall in the population of intravenous drug addicts. In this population acute disease may be manifested by IgG seroconversion with or without positive IgM. (Extract from author's abstract)
Etude du dépistage des anticorps anti-Leishmaniose chez des patients UDVI suspectés de Leishmaniose viscerale, présentant des troubles fébriles et des adénopathies, comparativement à un groupe de sujets sains non UDVI. Les deux techniques utilisées sont d'une part, l'immunofluorscence indirecte pour détecter les anticorps IgG et IgM et d'autre part, le test Elisa pour l'analyse des anticorps IgG. Dans ce diagnostic indirecte de la Leishmaniose chez les UDVI les deux méthodes sont complémentaires, la séroconversion IgG peut se manifester lors d'affection aiguë sans s'accompagner d'une positivité des IgM.
ENGLISH :
The prevalence of antibodies anti-Leishmania in intravenous drug addict (IVDA) patients suspect of visceral leishmaniasis (VL) and the diagnostic profitability of two tests for detecting anti-Leishmania antibodies were studied. METHODS : Serum samples corresponding to 91 healthy non IVDA individuals (group 1), 143 IVDA individuals attended in consultation for febrile illness and lymph node enlargement (group 2) with only one serum determination, and 42 IVDA individuals in whom sequential serologic study (group 3) was performed were analyzed. IgG and IgM antibodies were studied by indirect immunofluorescence (IIF). The individuals of group 1 and 90 of those of group 2 (45 with anti-Leishmania IgG antibodies and 45 without by IIF) were simultaneously studied by ELISA to analyze IgG antibodies. It was found that indirect immunofluorescence and ELISA techniques are complementary methods in the indirect diagnosis of visceral leishmaniasis overall in the population of intravenous drug addicts. In this population acute disease may be manifested by IgG seroconversion with or without positive IgM. (Extract from author's abstract)
Affiliation :
Dept Microbiol. , Hosp. Univ. San Cecilio, Univ. Granada, Espana
Cote :
A04208