Historique
Périodique
HIV-1 genetic diversity in Galicia spain: BG intersubtype recombinant viruses circulating among injecting drug users
(Diversité génétique du VIH-1 en Galicie espagnole : circulation de virus recombinants d'inter-sous type BG parmi les usagers de drogue injectable.)
Auteur(s) :
THOMSON, M. M. ;
DELGADO, E. ;
MANJON N. ;
OCAMPO A. ;
VILLAHERMOSA M. L. ;
MARINO A. ;
HERRERO I. ;
CUEVAS M. T. ;
VASQUEZ-DE PARGA E. ;
PEREZ-ALVAREZ, L. ;
MEDRANO L. ;
TABOADA, J. A. ;
NAJERA R. ;
THE SPANISH GROUP FOR ANTIRETROVIRAL STUDIES IN GALICIA
Année
2001
Page(s) :
509-516
Langue(s) :
Anglais
Domaine :
Drogues illicites / Illicit drugs
Discipline :
MAL (Maladies infectieuses / Infectious diseases)
Thésaurus mots-clés
VIH
;
INJECTION
;
CONTAMINATION
;
GENETIQUE
;
EPIDEMIOLOGIE DESCRIPTIVE
Thésaurus géographique
ESPAGNE
Note générale :
AIDS, 2001, 15, (4), 509-516
Résumé :
FRANÇAIS :
En Europe de l'Ouest, les virus de sous type B sont principalement responsables de l'épidémie à VIH-1, les autres sous types étant rares. Chez 451 personnes (de 7 villes de Galicie), les séquences ARN des sous types ont été déterminées selon l'algorithme de Blast. 4,4% de virus non de sous type B, avec une grande diversité de formes génétiques, ont été identifiés. 12 des infections, non dues à des virus de sous type B (soit 60%) concernaient les usagers de drogues injectables, qui pour la plupart n'avaient connaissance d'aucune source de contamination hors de l'Europe.
ENGLISH:
Background: The HIV-1 epidemics in Western Europe are dominated by B subtype viruses. Non-B subtype is largely restricted to individuals infected outside of Europe and to their direct contacts and is generally acquired by the heterosexual route. Methods: Protease and a segment of reverse transcriptase were amplified and sequenced from plasma RNA in 451 individuals from seven cities of Galicia, north- western Spain. Subtype sequence homologies were determined using the BLAST algorithm. Non-B sequences were examined by phylogenetic analysis and intersubtype recombination by bootscanning. The env V3 region was analysed in all non-B and in 38 B subtype viruses. Results: Ten different non-B genetic forms were identified in 20 (4.4%) individuals. Subtypes were concordant between pol and V3 in five viruses; 14 (70%) infections were with intersubtype recombinant viruses, and one individual had a dual B+G infection. Seven recombinant viruses were phylogeneticaliy related to five reported recombinant forms. Three non-recombinant G and six recombinant BG viruses formed a monophyletic cluster for pol. All but three individuals with non-B infections were native Spanish. Only 6 of 1 6 individuals referred to sexual contacts with sub-Saharan Africans. Twelve (60%) non-B subtype infections, including all with G and BG viruses, were in injecting drug users (IDU). Conclusions: Non-B subtype viruses were identified in 4.4%, with a high diversity of genetic forms, including 70% infections with intersubtype recombinant viruses. The majority of individuals with non-B infections were IDU, most of them without known contacts with non-European sources, and among whom BG recombinant viruses are circulating. (Author' s abstract)
En Europe de l'Ouest, les virus de sous type B sont principalement responsables de l'épidémie à VIH-1, les autres sous types étant rares. Chez 451 personnes (de 7 villes de Galicie), les séquences ARN des sous types ont été déterminées selon l'algorithme de Blast. 4,4% de virus non de sous type B, avec une grande diversité de formes génétiques, ont été identifiés. 12 des infections, non dues à des virus de sous type B (soit 60%) concernaient les usagers de drogues injectables, qui pour la plupart n'avaient connaissance d'aucune source de contamination hors de l'Europe.
ENGLISH:
Background: The HIV-1 epidemics in Western Europe are dominated by B subtype viruses. Non-B subtype is largely restricted to individuals infected outside of Europe and to their direct contacts and is generally acquired by the heterosexual route. Methods: Protease and a segment of reverse transcriptase were amplified and sequenced from plasma RNA in 451 individuals from seven cities of Galicia, north- western Spain. Subtype sequence homologies were determined using the BLAST algorithm. Non-B sequences were examined by phylogenetic analysis and intersubtype recombination by bootscanning. The env V3 region was analysed in all non-B and in 38 B subtype viruses. Results: Ten different non-B genetic forms were identified in 20 (4.4%) individuals. Subtypes were concordant between pol and V3 in five viruses; 14 (70%) infections were with intersubtype recombinant viruses, and one individual had a dual B+G infection. Seven recombinant viruses were phylogeneticaliy related to five reported recombinant forms. Three non-recombinant G and six recombinant BG viruses formed a monophyletic cluster for pol. All but three individuals with non-B infections were native Spanish. Only 6 of 1 6 individuals referred to sexual contacts with sub-Saharan Africans. Twelve (60%) non-B subtype infections, including all with G and BG viruses, were in injecting drug users (IDU). Conclusions: Non-B subtype viruses were identified in 4.4%, with a high diversity of genetic forms, including 70% infections with intersubtype recombinant viruses. The majority of individuals with non-B infections were IDU, most of them without known contacts with non-European sources, and among whom BG recombinant viruses are circulating. (Author' s abstract)
Affiliation :
Ctro Nacional Biologia Fundamental, Insto Salud Carlos III, Ctra Majadahonda-Pozuelo, 28220 Majadahonda (Madrid)
Espagne. Spain.
Espagne. Spain.